![]() Using the line selection tool, draw a line along the length of the feature or scale bar.If you know the size of a feature (for example, a previously applied scale bar) you can use this command to apply a calibration. In the new dialog, enter a value by which the receiving image has been scaled.In the “ from" drop-down box select the original image in the “ to" box, select the processed image.Open the image with the original calibration.There are three ways to reapply spatial calibration: manually via the “ Image/Properties” dialog using the "Copy Pixel Size" plugin that applies pixel size of one image to a second image or with the "Set Scale" plugin.Įrror creating thumbnail: Unable to save thumbnail to destination In this case, use the original file's spatial calibration (located under " Image/Properties") to update the " Image/Properties" values of the final, processed image. Sometimes the spatial calibration may be lost after applying an image processing function. The " Microscope Scale" plugin is another alternative and can be customized using a drop-down box of objectives to apply spatial calibrations. Spatial calibration can also be found and set in the " Image/Properties" dialog. Then the calibration can be applied using " Analyze/Set Scale" (see below). These settings include camera binning, frame size, objective, confocal zoom, etc. In the case that the files have no calibration, you can apply the calibration using images of a stage micrometer with the same settings as your experiment. Zeiss zvi's exported as TIFFs) will lose their calibration information completely. Perkin Elmer) are not calibrated while some "exported" image files (e.g. However, it is important to keep in mind that some file formats (e.g. Some microscope models (e.g., Biorad PIC, and Zeiss LSM confocal image files) will use the zoom and objective magnification settings for internal calibration. Note that the units "um" will automatically change to “µm”. A standard size should be used for the scale bars on all images if possible to help avoid confusion.Īn image's spatial calibration can be edited using " Image/Properties". Please contact the moderators of this subreddit if you have any questions or concerns.All images for publications should include a scale bar. I am a bot, and this action was performed automatically. If someday your work gets published, show it off here! That's one use of the "Research" post flair. "Time is the most precious gift in our possession, for it is the most irrevocable." ~ DB Remember that "free help" costs those who help:Īside from Automoderator, those responding to you are real people, giving up some of their time to help you. Karma is a small way to say "thanks" and "this was helpful". Upvote those who contribute to the discussion. (See: xkcd 979)Ĭonsider saying "thank you" in comment replies to those who helped. People from the future may be stuck trying to answer the same question. If you figure out the answer for yourself, please post it! Never delete your post, even if it has not received a response.ĭon't switch over to PMs or email. Respond when helpful users ask follow-up questions, even if the answer is "I'm not sure". Image analysis is interdisciplinary, so the more general the terminology, the more people who might be able to help.īe thorough in outlining the question(s) that you are trying to answer.Ĭlearly explain what you are trying to learn, not just the method used, to avoid the XY problem. Good places to upload include:, , & Īvoid discipline-specific terminology ("jargon"). Screenshots (to help identify issues with tools or features) Reference Images (taken from published papers)Īnnotated Mock-ups (showing what features you are trying to measure) Images give everyone a chance to understand the problem.Įxample Images (what you want to analyze) Notes on Quality Questions & Productive Participation
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